Biopolym. Cell. 2016; 32(3):203-221.
Геномика, транскриптомика и протеомика
Транскрипционная и постранскрипционная регуляция гена адапторного/скафолдного белка ITSN1
1Кропивко С. В., 1Губарь О. С., 1Грязнова Т. А., 1Мордерер Д. Е., 1Герасимчук Д. А., 2Сивак Л. А., 2Грабовой А. Н., 1Рындич А. В.
  1. Институт молекулярной биологии и генетики НАН Украины
    ул. Академика Заболотного, 150, Киев, Украина, 03680
  2. Национальный институт рака
    ул. Ломоносова, 33/43, Киев, Украина, 03022

Abstract

ITSN1 – адапторный/скафолдный белок который принимает участие в различных физиологических и патологических клеточных процессах. Он имеет сложное регулирование экспрессии и многочисленные белки партнеры. Цель. Характеристика функционирования и регуляция экспрессии ITSN1 имеет важное значение для понимания роли ITSN1 в жизни клетки. Методы. Биоинформатический анализ, полуколичественный анализ экспрессии с помощью ОТ-ПЦР, иммунопреципитация. Результаты. Описано и проанализировано промоторные области ITSN1, детектировано альтернативно сплайсированные изоформы ITSN1 на уровне мРНК и белка в разных образцах рака. С помощью различных биоинформатических серверов определены сайты связывания с микроРНК, также проанализированы сайты серин-, треонин- и тирозин-фосфорилирования белка ITSN1. Выводы. Получены новые данные о экспрессии ITSN1 при патологиях. Показана возможность регуляции экспрессии ITSN1 с помощью микроРНК и потенциальной роли фосфорилирования серина, треонина и тирозина в регуляции взаимодействия ITSN1 с белками партнерами.
Keywords: ITSN1, двунаправленный промотор, альтернативный сплайсинг, микроРНК, фосфорилирование

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