Biopolym. Cell. 1987; 3(5):270-274.
Gene-Engineering Biotechnology
Cloning of the Zea maize genome DNA sequences capable of autonomous replication in Saccharomyces cerevisiae
1Shulzhenko V. N., 1Kordyum V. A.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

DNA fragments of maize genome functioning as autonomously replicating sequences (ARS) in yeast were inserted into EcoRI-site of plasmid pYF40 bearing the His3 marker of yeast Saccharomyces cerevisiae. Two plasmids with inserts were selected which transformed strain His- S. cerevisiae LL20(leu2-3leu2-llhis3-llhis3-15) into His+ with a frequency of (1-3)·102 transformants per 1 u.g of plasmid DNA. ARS-plasmids are located in yeast cells in a nonintegrated form, that is confirmed by the isolation of plasmid DNA from them, with which both E. coli and S. cerevisiae cells are transformed. Size of cloned EcoRI DNA fragments is about 0.8 MD.

References

[1] Gorman JA, Dove WF, Warren N. Isolation of Physarum DNA segments that support autonomous replication in yeast. Mol Gen Genet. 1981;183(2):306-13.
[2] Loppes R, Denis C. Chloroplast and nuclear DNA fragments from Chlamydomonas promoting high frequency transformation of yeast. Curr Genet. 1983;7(6):473-80.
[3] Montiel JF, Norbury CJ, Tuite MF, Dobson MJ, Mills JS, Kingsman AJ, Kingsman SM. Characterization of human chromosomal DNA sequences which replicate autonomously in Saccharomyces cerevisiae. Nucleic Acids Res. 1984;12(2):1049-68.
[4] Roth GE, Blanton HM, Hager LJ, Zakian VA. Isolation and characterization of sequences from mouse chromosomal DNA with ARS function in yeasts. Mol Cell Biol. 1983;3(11):1898-908.
[5] Skatrud PL, Queener SW. Cloning of a DNA fragment from Cephalosporium acremonium which functions as an autonomous replication sequence in yeast. Curr Genet. 1984;8(3):155-63.
[6] Uchimiya H, Ohtani T, Ohgawara T, Harada H, Sugita M, Sugiura M. Molecular cloning of tobacco chromosomal and chloroplast DNA segments capable of replication in yeast. Mol Gen Genet. 1983; 192(1-2):1-4.
[7] Storms RK, McNeil JB, Khandekar PS, An G, Parker J, Friesen JD. Chimeric plasmids for cloning of deoxyribonucleic acid sequences in Saccharomyces cerevisiae. J Bacteriol. 1979;140(1):73-82.
[8] Clewell DB, Helinski DR. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969;62(4):1159-66.
[9] Birnboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979;7(6):1513-23.
[10] Miroshnichenko GP, Dyachenko AF Modern methods of DNA extraction from higher plants. Usp Sovrem biol. 1981; 91(1):49-60.