Biopolym. Cell. 1987; 3(3):131-134.
Short Communications
The interaction of different conformers of deacylated tRNA with 80S ribosomes
1Negrutsky B. S., 1Elskaya A. V.
  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR
    Kiev, USSR

Abstract

Biologically inactive tRNA conformers are able to inhibit the poly(U)-dependent binding of phenylalanyl-tRNA to 80S ribosomes, being a more efficient inhibitor than native deacylated tRNA.

References

[1] Matsuka GKh, Babii TP, Skvirskaia EB, Ovcharenko GV, Semenikhin VI. Biologically inactive transfer RNA in animal livers. Biokhimiia. 1973;38(6):1221-7.
[2] Demidov SV, Kovalenko MI, El'skaia AV. Biological activity of hepatic transfer RNA of rabbits of different ages. Nauchnye Doki Vyss Shkoly Biol Nauki. 1980;(12):18-24.
[3] Kovalenko MI, Rodovichius GA, Tamulyavichius AI, Pivoriunayte Study of the molecular basis of disorders of protein biosynthesis in experimental myocardial infarction and myocardial autolysis. Molekulyarnaya biologiya. 1984; Is. 37:18-21
[4] Negrutskii BS, Soldatkin KA. Thermodynamic assessment of conformational transitions upon renaturation biologically inactive tRNA conformers. Molekulyarnaya biologiya. 1984; Is. 37:26-8
[5] Negrutskii BS, El'skaia AV. Characteristic features of heat inactivation of leucyl-tRNA- synthetase from the rabbit liver in the presence of various tRNA conformers. Mol Biol (Mosk). 1984;18(5):1297-300.
[6] Sarin PS, Zamecnik PC. On the stability of aminoacyl-S-RNA to nucleophilic catalysis. Biochim Biophys Acta. 1964;91:653-5.
[7] Ovcharenko GV, Potapov AP, NegrutskiД­ BS, Soldatkin AP, Ven'iaminova AG. Code correspondence of tRNK Leu 1-6 in the mammary gland of cows. Ukr Biokhim Zh. 1983;55(6):608-13.
[8] Kovalenko MI, Zheltovskaya NI, Elkaya AV, Ovcharenko GV. Methods of isolation and individual tRNA and tRNKLeu tRNKPhe from animal tissues. Metody sovremennoy biologii. Kiev, Naukova dumka, 1979; 98-111
[9] Nirenberg M, Leder P. RNA codewords and protein synthesis. the effect of trinucleotides upon the binding of sRNA to ribosomes. Science. 1964;145(3639):1399-407.
[10] Wurmbach P, Nierhaus KH. Codon-anticodon interaction at the ribosomal P (peptidyl-tRNA)site. Proc Natl Acad Sci U S A. 1979;76(5):2143-7.
[11] Peters M, Yarus M. Transfer RNA selection at the ribosomal A and P sites. J Mol Biol. 1979;134(3):471-91.
[12] Ofengand J, Henes C. The function of pseudouridylic acid in transfer ribonucleic acid. II. Inhibition of amino acyl transfer ribonucleic acid-ribosome complex formation by ribothymidylyl-pseudouridylyl-cytidylyl-guanosine 3'-phosphate. J Biol Chem. 1969;244(22):6241-53.
[13] Kirillov SV, Makhno VI, Odinzov VB, Semenkov YP. The mechanism of codon-anticodon interaction in ribosomes. Heterogeneity of tRNA complexes with 70-S ribosomes of Escherichia coli. Eur J Biochem. 1978;89(1):305-13.