Biopolym. Cell. 2009; 25(4):266-271.
Molecular and Cell Biotechnologies
Reduction of chromate and carotene-synthesizing activity of selenite-resistant mutants of the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma)
1, 2Nechay H. I., 1Ksheminska H. P., 2Kolisnyk H. V., 3Grzadka M., 1, 3Gonchar M. V.
  1. Institute of Cell Biology, NAS of Ukraine
    14/16, Drahomanov Str., Lviv, Ukraine, 79005
  2. Institute of Animal Biology, NAAS of Ukraine
    38, Stusa Str., Lviv, Ukraine, 79034
  3. Zaklad Biotechnologii, Zamiejscowy Wydzial Biotechnologii, Uniwersytet Rzeszowski
    Kolbuszowa, Polska

Abstract

The yeast P. rhodozyma is a perspective microbial producer of carotenoid pigment astaxanthin with a high antioxidant power. The aim of the work was to study the ability of the selenite-resistant strains of this yeast to reduce chrome(VI) compounds, as well as to analyze the relations between synthesis of carotenoids, resistance to selenite and chromate-reducing activity of P. rhodozyma. Methods. The yeast cells were grown at standard conditions for this species. The residual chromate content in cultural liquid was determined colorimetrically using diphenylcarbazide. The carotenoid content was determined after extraction of the pigments from the previously permeabilized cells by organic solvents. Results. The selected selenite-resistant mutants of the yeast P. rhodozyma revealed the different combinations of the phenotypes related with tolerance/sensitivity to chromate and selenite, as well as ability to reduce chromate. Conclusions. The obtained results give reasons for suggesting that pathways of detoxification of chromate and selenite by the yeast P. rhodozyma are different, although run through a common reductive type. The isolated mutant strains would be served as the useful models to study relations between homeostasis of Se and Cr oxyanions and biosynthesis of carotenes.
Keywords: chromate, reduction, selenite, carotenes, Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

References

[1] Cherest H., Davidian J., Thomas D., Benes V., Ansorge W., Surdin-Kerjan V. Molecular characterization of two high affinity sulfate transporters in Saccharomyces cerevisiae. Genetics. 1997; 145(3):627–635.
[2] Breton A., Surdin-Kerjan V. Sulfate uptake in Saccharomyces cerevisiae: biochemical and genetic study. J. Bacteriol. 1977; 132(1):224–232.
[3] Camargo F. A. O., Bento F. M., Okeke B. C., Frankenberger W. T. Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate J. Environ. Qual 2003 32, N 4:1228–1233.
[4] Mabrouk M. E. M. Statistical optimization of medium components for chromate reduction by halophilic Streptomyces sp. MS-2. Afr. J. Microbiol. Res. 2008; 2:103–109.
[5] Baldi F., Vaghan A. M., Olson G. Chromium (VI)-resistant yeast isolated from a sewage treatment plant receiving tannery wastes. Appl. Environ Microbiol. 1990; 56(4):913–918.
[6] Ksheminska H. P., Honchar T. M., Gayda G. Z., Gonchar M. V. Extra-cellular chromate-reducing activity of the yeast cultures Cent. Eur. J. Biol 2006 1, N 1:137–149.
[7] Kaminska M., Solohub L. The carotenoproduced yeast Phaffia rhodozyma. Visnyk of L'viv Univ., Biology Series. 2004; 37:3–12.
[8] Higuera-Ciapara I., Felix-Valenzuela L., Goycoolea F. M. Astaxanthin: a review of its chemistry and applications Crit. Rev. Food. Sci. Nutr 2006 46, N 2:185–196.
[9] Verdoes J. C., Sandmann G., Visser H., Diaz M., Mossel M., Ooyen A. Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) Appl. Environ Microbiol 2003 69, N 7:3728–3738.
[10] Han R., Tian Y., Wu Y., Wang P., Ai X., Zhang J., Skibsted L. Mechanism of radical cation formation from the excited states of zeaxanthin and astaxanthin in chloroform Photochem. Photobiol. 2006; 82, N 2:538–546.
[11] Park D., Park J. M., Yun Y. S. Mechanisms of the removal of hexavalent chromium by biomaterials or biomaterial-based activated carbons J. Hazard. Mater 2006 137, N 2:1254–1257.
[12] Nechai H. Isolation spontaneous mutants of carotene-synthesizing yeast Phaffia rhodozyma which are resistant to sodium selenite. Abstr. II Int. Conf. of Young Scientist «Biology: from molecular to biosphere» (19–21 Nov. 2007, Kharkiv) Kharkiv, 2007:386.
[13] Sedmak J. J., Weerasinghe D. K., Jolly S. O. Extraction and quantitation of astaxanthin from Phaffia rhodozyma. Biotech. Tech. 1990; 4(2):107–112.
[14] Rahman M. U., Gul S., Ul Haq M. Z. Reduction of chromium(VI) by locally isolated Pseudomonas sp. C-171. Turk. J. Biol. 2007; 31:161–166.
[15] Banszky L., Simonics T., Maraz A. Sulphate metabolism of selenate-resistant mutants Schizosaccharomyces pombe J. Gen. Appl. Microbiol 2003 49:271–278.
[16] Birringer M., Pilawa S., Flohe L. Trends in selenium biochemistry Nat. Prod. Rep 2002 19:693–718.
[17] Chaban L., Pokrovetcka O., Stenchuk M., Gonchar M. Isolation and physiological characterization of selenite-resistant mutants of the yeast Pichia guilliermondii. Visnyk of L'viv Univ., Biology Series. 2004; 34:92–99.
[18] Ksheminska H., Fedorovich D., Honchar T., Ivash M., Gonchar M. Yeast tolerance to chromium depends on extracellular chromate reduction and Cr(III)-chelation. Food Technol. Biotechnol. 2008; 46(4):420–427.
[19] Ni H., Chen Q., Ruan H., Yang Y., Li L., Wu G., Hu W., He G. Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma J. Zhejiang Univ. Sci. B 2007 8, N 5:365–370.
[20] Ksheminska H., Honchar T., Usatenko Yu., Gonchar M. Extra-cellular chromate-reducing activity of the yeast cultures Abstr. II Polish-Ukrain. Weigl Conf. «Microbiology in the XXI century» (24–26 Sept. 2007, Warsaw) Warsaw, 2007:240.
[21] Gayda G., Ksheminska H., Prokopiv T., Ivash M., Nechay G., Usatenko Y., Gonchar. M. Extra-cellular reduction in chromate detoxification by daker's and non-conventional yeasts: Study of the mechanisms of Cr(III)-biochelates generation and their characterization Abstr. XII Int. Congr. on Yeasts (11–15 Aug., 2008, Kyiv) Kyiv, 2008:197.