Biopolym. Cell. 1986; 2(2):81-87.
http://dx.doi.org/10.7124/bc.0001A0
Structure and Function of Biopolymers
Stability of copper (II) complexes with denatured DNA in solutions of various ionic strength
1Baby A. P., 1Bagaveev I. A., 1Uminsky A. A., 1Saenko G. N.
  1. Institute of Chemistry of the Far-East Research Centre, Academy of Sciences of the USSR
    Vladivostok, USSR

Abstract

Conditions necessary for the formation and stability of Cu(II) complexes with the denatured DNA (dDNA) in solutions of various ionic strength are studied by the electron spectroscopy method. A chelate nature of the spectroscopically registered Cu(II) — dDNA complex in which Cu(II) ions form a bond with nitrogen base and phosphate group of biopolymer is experimentally confirmed. The «true» and seeming stability constants of the formed complexes are calculated. The possible structure of Cu(II) — dDNA complexes is suggested.
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References

[1] Eichhorn GL, Shin YA. Interaction of metal ions with polynucleotides and related compounds. XII. The relative effect of various metal ions on DNA helicity. J Am Chem Soc. 1968;90(26):7323-8.
[2] Kriss EE, Yatsimirskii KB. Reaction of nucleic acids with metals. Russ Chem rev, 1966; 35 (2):144–152.
[3] Kunkel TA, Loeb LA. On the fidelity of DNA replication. Effect of divalent metal ion activators and deoxyrionucleoside triphosphate pools on in vitro mutagenesis. J Biol Chem. 1979;254(13):5718-25.
[4] Popenoe EA, Schmaeler MA. Interaction of human DNA polymerase beta with ions of copper, lead, and cadmium. Arch Biochem Biophys. 1979;196(1):109-20.
[5] Veselkov AN, Moroshkin VA, Poliakova ID, Shpungin IL, Frisman ZV. Conformation of the denatured DNA molecule in solutions of different ionic strengths. Mol Biol (Mosk). 1976;10(5):1050-60.
[6] Popov LS. Single stranded DNA structure in prokaryotes and eukaryotes. Uspekhi sovremennoy biologii. 1984; 97(1):83-100.
[7] Hardy KJ, Bryan SE. Localization and uptake of copper into chromatin. Toxicol Appl Pharmacol. 1975;33(1):62-9.
[8] Yatsimirskiy KB, Babiy AP, Mashkovtsev RI. Investigation of the interaction of divalent copper with deoxyribonucleic acid by EPR spectroscopy. Theoretical and Experimental Chemistry. 1984; 20(1):105-10.
[9] Spirin AS. Spectrophotometric determination of total nucleic acids. Biokhimiia. 1958;23(5):656-62.
[10] Blagoi IuP, Sorokin VA, Valeev VA. Spectral studies of binding between DNA bases and magnesium or calcium ions. Mol Biol (Mosk). 1980;14(3):595-605.
[11] Yatsimirskiy KB, Kriss EYe, Akhrameyeva TI. Study of complexation of copper ions with deoxyribonucleic acid. Dokl akad nauk SSSR. 1966; 168(4):840-3.
[12] Higasi K, Baba H, Rembaum A. Quantum Organic Chemistry. New York: Wiley, 1965; 358 p.
[13] Bregadze VG. Interpretation of ultraviolet difference spectra of DNA in complexes with ions of the 1st transitional series. Biofizika. 1974;19(1):179-81.
[14] Yatsimirskiy KB, Kriss EYe, Gvyazdovskaya VL. Stability constants of complexes of metals with bioligands. Kiev, Naukova Dumka, 1979. 226 p.
[15] Zimmer C, Luck G, Triebel H. Conformation and reactivity of DNA. IV. Base binding ability of transition metal ions to native DNA and effect on helix conformation with special reference to DNA-Zn(II) complex. Biopolymers. 1974;13(3):425-53.
[16] Daune M. Binding of divalent cations to DNA. Stud biophys. 1970; 24(25):287-297.
[17] Lur'ye YuYu. Handbook of Analytical Chemistry. Moscow, Khimiya, 1979; 480 p.
[18] Studier FW. Effects of the conformation of single-stranded DNA on renaturation and aggregation. J Mol Biol. 1969;41(2):199-209.
[19] Hiai S. Effects of cupric ions on thermal denaturation of nucleic acids. J Mol Biol. 1965;11:672-90.
[20] Forster W, Bauer E, Schutz H, Berg H, Akimenko M, Minchenkova LE, Evdokimov YuM, Varshavsky IaM. Thermodynamics and kinetics of the interaction of copper (II) ions with native DNA. Biopolymers. 1979;18(3):625-61.
[21] Felsenfeld G, Hirschman SZ. A neighbor-interaction analysis of the hypochromism and spectra of DNA. J Mol Biol. 1965;13(2):407-27.
[22] Deb KK. Ultraviolet Spectroscopic Study of Cu2+ Induced DNA Melting at Room Temperature. Spectrosc. Lett. 1981; 14(5):385-93.