Білковий блотинг
DOI:
https://doi.org/10.7124/bc.0001BDАнотація
Метод білкового блотингу активно використовується в молекулярно-біологічних дослідженнях для аналізу взаємодій типу білок–ДНК і білок–білок, включаючи визначення специфічності антигенів і антитіл. Розглянуто різні етапи блотингу – перенесення білків на мембрани, обробка блотів, створення імунних комплексів на репліках і фарбування блотів. Представлено два приклади застосування імуноблотингу для аналізу специфічності антитіл до інтерферонів людини.Посилання
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