Phycobiliprotein pigments for biomolecules. 1. Preparative fractionation and spectral characteristics

Authors

  • V. Y. Chernenko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. V. Kiryushyn Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • L. L. Lukash Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.00043D

Abstract

The authors have elaborated technological approaches concerning preparative fractionation of phycobiliproteins – phycoerythrin from a microalga Porphyridium cruentum, and of phycocyanin from a cyanophyte alge Anabaena variabilis. The spectral properties of dyes obtained have been also investigated.

References

Oi VT, Glazer AN, Stryer L. Fluorescent phycobiliprotein conjugates for analyses of cells and molecules. J Cell Biol. 1982;93(3):981-6.

Cordier G. Flow cytometry for immunology. Biol Cell. 1986;58(2):147-50.

Antibodies, A practical approach. Ed Catty D. 1989 IRL Press Oxford. 259p

Andreyuk EI, Kopteva ZhP, Zanina VV Cyanobacteria. K.: Naukova Dumka, 1990. 197 p.

AS. N 1400275 (USSR). Separation method a1-antitrypsin in the fraction. GG Demidov, V. Yu Chernenko, KA Odynets, LE Korol.

Troitskiy GV, Azhitskiy BYu. Isoelectric focusing of proteins in self-organizing and artificial pH gradients. Kiev: Naukova Dumka, 1984. 215 p.

Paster EU, Ovod VV, Pozur VK, Vikhot' NE. Immunologiya. Prakticum. K. : Visha shk., 1989; 128-30.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.

Patent USA N 4 859 582 Fluorescent conjugates for analysis of molecules and cells. L. Stryer, A. Glazer, V. Oi. 1989.

Published

1996-07-20

Issue

Section

Biopolymers and Cell