Lysogeny by MS2 phage. Analysis of a recombinant plasmid containing MS2 RNA-like sequence

Authors

  • T. P. Pererva Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • N. Yu. Mirjuta Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. Yu. Mirjuta Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • M. I. Woodmaska Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • E. N. Zherebtsova Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.0003D4

Abstract

pL34 recombinant plasmid coding MS2-llke RNA synthesis in plasmid-containing cells has been studied using physical mapping and blot-hybridisation with P32-labelled pMS27 plasmid fragments including a DNA copy of MS2 phage RNA. The authors have determined the size of a fragment containing MS2-like sequence and its localisation inside the hybrid plasmid.

References

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Published

1995-01-20

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Section

Biopolymers and Cell