Some differences between BamHl and Bnal restrictases with the same specifity

Authors

  • E. L. Kim Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • O. P. Makhovich Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • S. S. Maliuta Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author

DOI:

https://doi.org/10.7124/bc.000099

Abstract

The restriction endonuclease Bnal, being BamHl true isoshisomer, is isolated from B. natto B3364 strain. Its molecular weight, stability and optimal reaction conditions are studied. Comparative investigations have shown that according to these physicochemical characteristics the BamHl and Bnal enzymes differ from each other in spite of their analogous specificity.

References

Kessler C, Neumaier PS, Wolf W. Recognition sequences of restriction endonucleases and methylases--a review. Gene. 1985;33(1):1-102.

Wilson GA, Young FE. Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J Mol Biol. 1975;97(1):123-5.

Kim EL, Maliuta SS. BnaI - a new isoschizomer restriction enzyme BamHI. Biotekhnologiia. 1986; 4:24-7.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680-5.

Gooderham K. High-Sensitivity Silver Staining of Proteins Following Polyacrylamide Gel Electrophoresis. Proteins. 1984;113–8.

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Published

1989-01-20

Issue

Vol. 5 No. 1 (1989)

Section

Short Communications