Endonuclease Mval dimerization induced by the oligonucleotide substrate
DOI:
https://doi.org/10.7124/bc.000237Abstract
Molecular weight of the endonuclease Mval was determined by the method of gel electrophoresis under denaturing conditions. The enzyme consists of a single polypeptide chain with molecular weight of 31300±400 dalton. Similar data are obtained by gel filtration and sucrose density gradient centrifugation. In the presence of substrate the oligonucleotide molecular weight of endonuclease Mval increases up to 53000±3000 dalton, that is related to protein dimerization during the formation of the enzyme-substrate complex.References
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