Thermodynamics of the B-Z transition of poly[d(G-C)] in water/ethanol solution. The «tie» calorimetry
DOI:
https://doi.org/10.7124/bc.00021BAbstract
Poly[d(G–C)] in 55 % ethanol solution shifts from the Z form to the B form with the temperature rise from 20 to 50 °C. Enthalpy of the B-Z transition, ΔHBZ, has been determined with a «tie», a polyamine, which stabilized the Z conformation: ΔHBZ = –1.4 kcal/mol. This value was shown to be practically independent from the ionic strength in the range of 5·10–4–2·10–3 M NaCl.References
Karapetyan AT, Minyat EE, Ivanov VI. Increase in temperature induces the Z to B transition of poly[d(G-C)] in water-ethanol solution. FEBS Lett. 1984;173(1):243-6.
Pohl FM, Jovin TM. Salt-induced co-operative conformational change of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC). J Mol Biol. 1972;67(3):375-96.
Ivanov VI, Minyat EE. The transitions between left- and right-handed forms of poly(dG-dC). Nucleic Acids Res. 1981;9(18):4783-98.
Woisard A, Guschlbauer W, Fazakerley GV. The low ionic strength form of the sodium salt of poly(dm5C-dG) is a B DNA. Nucleic Acids Res. 1986;14(8):3515-9.
Pohl FM, Jovin TM, Baehr W, Holbrook JJ. Ethidium bromide as a cooperative effector of a DNA structure. Proc Natl Acad Sci U S A. 1972;69(12):3805-9.
Lazurkin YS, Frank-Kamenetskii MD, Trifonov EN. Melting of DNA: its study and application as a research method. Biopolymers. 1970;9(11):1253-306.
Frank-Kamenetskii MD, Karapetyan AT. Theory of the melting of DNA complexes with low-molecular substances. Mol Biol. 1972;6(4):500-4.
