Trp144 as a fluorescence probe for investigation of the C-module rapid conformation dynamics in eukaryotic tyrosyle-tRNA synthetase

Authors

  • M. A. Kordysh Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • K. A. Odynets Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. I. Kornelyuk Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.000671

Abstract

The spectra of fluorescence of the tyrosyle-tRNA synthetase C-module have been obtained. The microsurrounding of a tryptophan residue has been characterized using the methods of fluorescence spectroscopy and computational modeling. The rapid conformation dynamics of the C-module in nanosecond time scale is revealed using the fluorescence quenching with acrylamide and Cs ions.

References

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Published

2003-09-20

Issue

Vol. 19 No. 5 (2003)

Section

Structure and Function of Biopolymers