Phagedependent overproduction of β-galactosidase Escherichia coli and working out its purification

Authors

  • S. I. Chernykh Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • I. Yu. Slavchenko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • Yu. I. Gorlov Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. G. Terent'ev Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • T. G. Gavrish Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • V. A. Kordyum Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.0004C4

Abstract

The phagedependent method of obtaining of β-galactosidase (EC 3.2.1.23) E. coli has been worked out. The yield was up 700 U of enzyme activity per ml of cultural fluid. Accumulation of enzyme in cultural medium has permitted to use membrane method for preliminary purification of β-galactosidase. Concentrated and partially purified preparation of β-galactosidase has been purified by ligand-affinity chromatography. Pure preparation of β-galactosidase has characterized by high purity and nativeness. The process of obtaining and purification of β-galactosidase were made in pilot scale.

References

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Published

1998-03-20

Issue

Vol. 14 No. 2 (1998)

Section

Structure and Function of Biopolymers