Urease-based biosensor with improved sensitivity for heavy metal ions analysis
DOI:
https://doi.org/10.7124/bc.000499Abstract
Potcntiometric biosensors which consist of two ISFET structures covered with immobilized enzyme (urease) can be used for quantitative estimation, of water pollution with heavy metal ions. The measurements of the residual enzyme activity have been carried out in Tris-HCl buffer (pH 7,4) after preincubation in model CuCl2 solution. It was shown that sensor sensitivity to inhibitor concentration depends strongly on enzyme immobilization method. The lowest detection limit has been obtained for urease biosensor modified by negatively charged additional Nafion membrane.References
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