Associative clone selection of chinese hamster cells for a complex of quantitative characters
DOI:
https://doi.org/10.7124/bc.0001B5Abstract
Associative clone selection for a complex of quantitative characters was carried out in populations of cultivated cells. Two hypotetraploid cell populations of Chinese hamster of plus- and minus-direction of selection were studied. The possibility of preserving relatively stable caryotypic structure of polyploid cell populations of spontaneous origin during 20 cell passages. An entropy index was used as a measure of caryotypic heterogeneity and general structure indefinitness of genetic systems – cell populations. A definite correlation between an increase in the genome number in a cell and a change in a set of quantitative characters was determined.References
Harris M. Mutation rates in cells at different ploidy levels. J Cell Physiol. 1971;78(2):177-84.
Harris M. Polyploid series of mammalian cells. Exp Cell Res. 1971;66(2):329-36.
McBurney MW, Whitmore GF. Selection for temperature-sensitive mutants of diploid and tetraploid mammalian cells. J Cell Physiol. 1974;83(1):69-74.
Petrova ON, Manuilova ES, Shapiro NI. Hybridization of Chinese hamster cells sensitive to ultraviolet irradiation. Genetika. 1977;13(4):637-45.
Morrow J, Stocco D, Barron E. Spontaneous mutation rate to thioguanine resistance is decreased in polyploid hamster cells. J Cell Physiol. 1978;96(1):81-6.
Thompson KV, Holliday R. The longevity of diploid and polyploid human fibroblasts. Evidence against the somatic mutation theory of cellular ageing. Exp Cell Res. 1978;112(2):281-7.
Brodskiy V, Uryvayeva IV. Cell polyploidy: Proliferation and differentiation. Moscow, Nauka, 1981; 260 p.
Iashvili GD, Chernikov VG, Pichugina EM. Studying activity of nucleolar organizer regions of chromosomes in Chinese hamster clones different ploidy. Bull Exp Biol Med. 1983; 95(3):79-81.
Savchenko VK. Associative selection and its role in the evolution and breeding. Zh Obshch Biol. 1980; 41(3):406-417.
Savchenko VK. Genetic analysis in network test crosses. Minsk, Nauka i tekhnika, 1984; 224 p.
Adams RLP. Cell Culture for Biochemists. Elsevier/North-Holland Biomedical Press. 1980; 292 p.
Puck TT, Marcus PI. Action of X-rays on mammalian cells. J Exp Med. 1956;103(5):653-66.
Moorhead PS, Nowell PC, Mellman WJ, Battips DM, Hungerford DA. Chromosome preparations of leucocytes cultured from human peripheral blood.Exp Cell Res. 1960;20:613-6.
Venttsel' ES. Probability. Moscow, Nauka, 1969; 358 p.
Vakhtin IuB, Ignatova TN, Fridlianskaia II, Shvemberger IN. Intensity of selection and rate of major karyotypic changes in the population of somatic cells during cloning. Tsitologiia. 1965;7(2):258-9.
Bakhtin YB. Genetic theory of cell populations. Leningrad, Nauka, 1980; 168 p.
Glebov OK, Abramian DS. Nature of the phenotypic variability of somatic cells in culture: unstable phenotypic changes. Tsitologiia. 1984;26(3):235-51.
