Optimization of obtaining the high label single strain probe based on the M13 phage

Authors

  • G. D. Telegeev Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • M. V. Dybkov Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • K. N. Kiyanitsa Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.0003F3

Abstract

A method is proposed to obtain high-label single strain probe on the basis of the M13 phage. This is based on the use of a specific primer and the procedure of purification of the synthesized probe using nitrocellulose.

References

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Ryskov AP, Dzhincharadze AG, Prosnik MI, Ivanov PL, Limborskaia SA. Genomic fingerprints of organisms from different taxonomic groups: the use of phage M13 DNA as a hybridization probe. Genetika. 1988;24(2):227-38.

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Westneat DF, Noon WA, Reeve HK, Aquadro CF. Improved hybridization conditions for DNA 'fingerprints' probed with M13. Nucleic Acids Res. 1988;16(9):4161.

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Published

1995-07-20

Issue

Section

Biopolymers and Cell