Studding of role site plasmin for relation with lysine and arginine on early stages fibrin(ogen) hydrolysis
DOI:
https://doi.org/10.7124/bc.0003F2Abstract
The effect of 6-aminohexane acid (6-AHA) and L-arginine on the rates of early stages of fibrinogen hydrolysis by Lys77-plasmin, Val442-plasmin and Lys530-plasmin were studded. It was shown that 6-AHA on the second reaction stage (hydrolysis of X) in the concentrations saturating the site, of plasmin heavy chain had a strong inhibiting influence even more stronger than a the first stage (hydrolysis of fibrinogen). Effect of arginine and 6-AHA on the first stage hydrolysis was identical. Arginine effect on the second stage failed to be observed while its concentration were effecting kringle 1 and 5. It was suggested the enzyme interaction with substrate took place on the first stage of hydrolysis under kringles 5 and 1 patrkipation on second stage – under kringle 4 participation.References
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