Preparation of the fluorescein reagent for solid-phase oligonucleotide 5'-labelling and its use for the synthesis of fluorescently labelled PCR primers for HIV-1 detection
DOI:
https://doi.org/10.7124/bc.0003EBAbstract
The preparative synthesis of fluorescein H-phosphonate reagent for solid phase 'labelling of oligonucteotides is described. Fluorescein-labelted thymidine and two PCR primers for HIV-1 detection were synthesized using this reagent.References
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