The transient character of nonspecific genome's reaction under gamma irradiation in vivo tested by variation of rat DNA restriction pattern
DOI:
https://doi.org/10.7124/bc.0003C7Abstract
At the considerable enzyme's abundance the accessibility some rat thymus DNA sites, for EcoRI-restriction varies depending on the interval after a single γ-irradiation in vivo. At a first hours for 5 Gy the sign-opposite effects for the fragments 1500 and 345 b. p. are occured-increasing and decreasing of the relative portions accordingly – with the returning to initial restriction level to 24-th hour. The doze 0,5 Gy causes a similar genome's reaction in terms of restriction accessibility, but the manifestation occured only in a day.References
Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
Lapeyre JN, Becker FF. Analysis of highly repeated DNA sequences of rat with EcoR1 endonuclease. Biochim Biophys Acta. 1980;607(1):23-35.
Pech M, Igo-Kemenes T, Zachau HG. Nucleotide sequence of a highly repetitive component of rat DNA. Nucleic Acids Res. 1979;7(2):417-32.
Lichina MV, Kostuk GV, Mitrochin YuI, Shugalii AV. The restriction analysis of thymus and spleen DNA of a rat γ-irradiated at dose 5 Gy. Biopolym Cell. 1994; 10(1):31-36.
Spitkovskiĭ DD, Zborovskaia IB, Kiselev FL. Transcription of cellular oncogenes in human tumors. Mol Biol (Mosk). 1986;20(5):1409-21.
Philippsen P, Streeck RE, Zachau HG. Defined fragments of calf, human, and rat DNA produced by restriction nucleases. Eur J Biochem. 1974;45(2):479-88.
Lilcke-Huhle C, Herrlich P. Radiation-induced amplification of viral DNA sequence integrated in the genome of Chinese hamster embryo cells. Int J Rad Biol. 1986; 49(4):702-3.
Lücke-Huhle C, Pech M, Herrlich P. Selective gene amplification in mammalian cells after exposure to 60Co gamma rays, 241Am alpha particles, or uv light. Radiat Res. 1986;106(3):345-55.
Fonarev AB, Shugaliĭ AV, Papina RI, Todorov IN. Structural characteristics of DNA from the rat liver during inhibition of protein synthesis with cycloheximide. Biokhimiia. 1985;50(7):1162-6.
Lichina MV, Fonarev AB, Sidorenko LI. et al. Amplification of genomic fragments under the action of in vivo protein synthesis inhibitor and hepatocarcinogen. Structure and function of the cell nucleus: IX All-Union. Symp. Chernogolovka, 1987: 233.
Coggle J. Biological effects of radiation. Ed. AN Dedenkova. Energoatomizdat, 1986. 184 p.
