Nucleotide sequence of MnNPV polyhedrin gene.

Authors

  • O. I. Petrenko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • L. I. Strokovskaya Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. P. Solomko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.0003B8

Abstract

We have constructed a baculovirus expression vector based on Malacosoma neustria nuclear polyhedrosis virus (MnNPV). The vector showed itself as an effective tool for the expression of foreign gene. Presented here is the complete nucleotide sequence of MnNPV polyhedrin gene which is used for the insertion of genes to be expressed.

References

Strokovskaya L. I., Veselovsky O. V., Kikhno I. M., Mirjuta N. Yu. Cloning of the Malakosoma neustria nuclear polyhedrosis virus polyhedrin gene. Biopolym Cell. 1990;6(3):81-84.

Rohrmann GF. Polyhedrin structure. J Gen Virol. 1986;67 (Pt 8):1499-513.

Luckow VA, Summers MD. Trends in the Development of Baculovirus Expression Vectors. BioTechnology. 1988;6(1):47–55.

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Published

1994-07-20

Issue

Section

Short Communications