Indirect determination of the 5'→3' and 3'→5' of the DNA-dependent DNA-polymerase function
DOI:
https://doi.org/10.7124/bc.0003B7Abstract
Doxorubicin in the concentration of 1 mg/ml and streptonigrin in the concentration 1000 mg/ml each agent separately should by useful to determination of replicative (5'→3') or reparatlve (3'→5') function DNA-polymerase. This enzymes with 5'→3' fully inhibited by doxorubicine but net sensitive to action of streptonigrin in concentration substances mentioned above. And contraverse, DNA-polymerase with 3'→5' function don't sensitive to doxorubicin and it's activity depressed by streptonigrin.References
Chuang RY, Chuang LF. Inhibition of chicken myeloblastosis RNA polymerase II activity by adriamycin. Biochemistry. 1979;18(10):2069-73.
Galf EF, Cundliff E, Reynolds PE. et at. The molecular basis of antibiotic action. Ed. A Wiley London; New York: John Wiley and Song, 1972. 500 p.
Bauer GA, Heller HM, Burgers PM. DNA polymerase III from Saccharomyces cerevisiae. I. Purification and characterization. J Biol Chem. 1988;263(2):917-24.
Kedinger C, Gniazdowski M, Mandel JL Jr, Gissinger F, Chambon P. Alpha-amanitin: a specific inhibitor of one of two DNA-pendent RNA polymerase activities from calf thymus. Biochem Biophys Res Commun. 1970;38(1):165-71.
Roeder RG. Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Isolation and partial characterization. J Biol Chem. 1974;249(1):241-8.
Byrnes JJ, Downey KM, Black VL, So AG. A new mammalian DNA polymerase with 3' to 5' exonuclease activity: DNA polymerase delta. Biochemistry. 1976;15(13):2817-23.
Rüttimann C, Cotorás M, Zaldívar J, Vicuña R. DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8. Eur J Biochem. 1985;149(1):41-6.
