MS2 phage lysogeny. Phage-specific RNA synthesis on cell DNA

Authors

  • T. P. Pererva Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • N. Yu. Miryuta Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. Yu. Miryuta Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.00034D

Abstract

A fragment of DNA that controls the basic phenotypic properties MS2-induced lysis mutants of E. coli 3000, has been cloned as part of a hybrid plasmid. In cells transformed with this plasmid accumulated MS2-specific RNA, indicating that the inclusion of a strong phage genetic material into the cell DNA.

References

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Published

1993-01-20

Issue

Section

Gene-Engineering Biotechnology