Cloning in the laclq of pTTQ plasmids: «reverse» colour reaction
DOI:
https://doi.org/10.7124/bc.000343Abstract
The method for cloning Mlul-, BclI-, ApaI-, BssHI- and EcoRV-DNA fragments in the laclq gene of pTTQ plasmids is described. Recombinant clones are selected as coloured in blue colour colonies on plates containing ampicillin and X-gal.References
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