Comparison of solid-phase A-protein immune electron microscopy, direct electron microscopy and dot-ELISA for detection of transmissible gastroenteritis virus in feces
DOI:
https://doi.org/10.7124/bc.000342Abstract
The authors have shown the solid-phase A-protein immune electron microscopy permits, to detect the fecal coronavirus with the highest sensitivity (54.9 % of 171 fecal samples studied) comparing to direct electron microscopic examination (39.1 %). It is possible to identifity transmissible gastroenteritis virus and to differ it from other virus particles. Dot-ELlSA method has shown to demonstrate viral antigen in 51.4 % of the 171 samples investigated. So the solid-phase immune electron microscopy and dot-ELLSA are convenient for the veterinary diagnostics.References
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