Plasmid DNA screening in strains of microorganisms used for cleaning of industrial waste water

Authors

  • T. N. Shevchenko Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • A. V. Roy A. V. Dumansky Institute of Colloid and Water Chemistry, NAS of Ukraine 42 Vernadsky blvd., Kyiv, Ukraine, 03680 Author
  • A. Yu. Miruta Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • N. A. Klimenko A. V. Dumansky Institute of Colloid and Water Chemistry, NAS of Ukraine 42 Vernadsky blvd., Kyiv, Ukraine, 03680 Author
  • T. P. Pererva Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.00033B

Abstract

A member of B. subtilis, Ps. alcaligenes, Ps. putida strains has been analyzed to detect plasmid DNAs. The authors have showed, the strains capable to utilize the surface-active substances to contain plasmid DNAs. The utilization of urea does not correlate with plasmid carrying and appears to be coded by chromosomal genes.

References

Hardy KJ. Plasmids: a practical approach. IRL Press, 1987. 180 p.

Spizizen J, Reilly BE, Evans AH. Microbial transformation and transfection. Annu Rev Microbiol. 1966;20:371-400.

Taranova AA, Ovcharov AF, Rotmistrov MN. Bacterial degradation of ampholytic surfactants. Biotekhnologiya. 1990; 4:31-3.

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Published

1992-09-20

Issue

Vol. 8 No. 5 (1992)

Section

Gene-Engineering Biotechnology