Replication of oncogene c-myc in Ehrlich ascitic cells

Authors

  • I. M. Danko R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine 45, Vasilkivska Str., Kyiv, Ukraine, 01022 Author
  • V. I. Prima Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.000331

Abstract

Sedimentational fractionation of asynchronic Ehrlich ascitic cells po¬pulation was carried out according to cell cycle phases. Separation of tumor cells suspension was carried out under 1 g in 0–0,3 M sucrose concentration gradient. Cells, pituated in S phase, were selected to 3 fractions in early, midle and late S phase respectively. It was studied temporal order replications of synchronized Ehrlich ascitic cells. It was shown that the replication of oncogene c-myc beginning in early S phase.

References

Iqbal MA, Chinsky J, Didamo V, Schildkraut CL. Replication of proto-oncogenes early during the S phase in mammalian cell lines. Nucleic Acids Res. 1987;15(1):87-103.

Calza RE, Eckhardt LA, DelGiudice T, Schildkraut CL. Changes in gene position are accompanied by a change in time of replication. Cell. 1984;36(3):689-96.

Kavetskiĭ RE, Kaz'min SD. Energetics of the mitotic cycle in Ehrlich tumor cells. Dokl Akad Nauk SSSR. 1973;212(5):1217-9.

Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.

Kelly K, Cochran BH, Stiles CD, Leder P. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983;35(3 Pt 2):603-10.

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Published

1992-07-20

Issue

Vol. 8 No. 4 (1992)

Section

Gene-Engineering Biotechnology