The effect of neomycin on poly(U) and poly(dT) translation in cell-free systems from Saccharomyces cerevisiae and Neurospora crassa
DOI:
https://doi.org/10.7124/bc.000328Abstract
The effect of different concentration of neomycin on poly(U) and poly(dT) translation has been studied in cell-free systems from S. cerevisiae and M. crassa. The data show that this translation is carried out by cytoplasmic ribosomes. Mg2+ iones stimulate poly(U) and poly(dT) translation in these systems and neomycin unlike the prokaryotic cell-free systems inhibits translation of both templates. It was proposed that ribosome decoding center was changing during evolution to higher stereospecificity to the codon-anticodon sugar-phosphate backbone structure.References
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