Uridine-diphosphate-N-Acetyl-muramyl-L-alanyl-d-glutamate: meso-2, 6-diaminopimelate ligase activity in extracts of Basillus subtilis, Streptococcus bovis and Enterococcus faecium

Authors

  • E. O. Kalcheva Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • M. M. Faiziev Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author
  • S. S. Maluta Institute of Molecular Biology and Genetics, NAS of Ukraine 150, Akademika Zabolotnoho Str., Kyiv, Ukraine, 03680 Author

DOI:

https://doi.org/10.7124/bc.000305

Abstract

Uridine-diphosphate-N-acetylmuramyl-L-alanyl-D-glutamate: meso-2, 6-diaminopimelate ligase activity was found in extracts of B. subtilis, S. bovis, E. faecium. UDP-N-acetylmuramyl-L-alanyl-D-glutamate: meso-2, 6-diaminopimelate ligase from B. subtilis was purified by gel-filtration and ion-exchange chromatography. The molecular weight of enzyme as determined by gel-filtration is 120000.

References

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Published

1991-11-20

Issue

Section

Structure and Function of Biopolymers