In vitro induced morphogenesis of walnut
DOI:
https://doi.org/10.7124/bc.0002E2Abstract
The ability of apical axillary buds of 15–30 and 1–3 years old walnut trees to morphogenesis in vitro was studied. Adventitious shoot formation was induced on MS- media containing IBA (0,01 mg/1) and 6-BAP (1 mg/l) after 12 weeks of cultivation (including two subcultivations after 2 and 6 weeks). Propagated shoots were pretreated in liquid MS-medium with IBA (1; 5; 10 mg/1) and sucrose (60 g/l) during 6 days in dark. Rooting of the walnut shoots was achieved on hormone free MS-medium (macro-salts were dissolved 1/4) with agar.References
Rybin VA methods of vegetative propagation of walnut. Kishinev: Shtiintsa, 1961. 29 p.
Tsurkan IP. Growing grafted walnut planting material in Moldova. Achievements in the fruit and nursery management NRB MSSR. Chisinau Maps Moldovenyaska. 1978:208-213.
Strela TE. New biotechnology walnut grafting. Kiev: Naukova Dumka, 1987. 59.
Chalupa V. Vegetativni mnozeni brizy (Betula pendula Roth.), dubu (Quercus robur L.) a jasanu (Fraxinus escelsior L.) in vitro. Pr. vulhm. 1983;62:179-94.
Pua Eng-Chong, Chong Calvin. Regulation of in vitro shoot and root regeneration in Macspur apple by sorbitol (Z)-glucitol) and related carbon sources. J Amer Soc Hort Sci. 1985; 110(5):705-9.
Driver JA, Kuniyuki AH. In vitro propagation of Paradox wal nut rootstock. Hort Sci. 1984; 19(4):507-9.
Meynier V. Mise en culture in vitro de meristeme de Noyers hybrioes. C r Acad Sci C. 1985; 301(5):261-4.
Wood BW. In vitro proliferation of pecan. Hort Sci. 1982; 17(6):890-1.
Liu S, Han B. In vitro propagation of walnut (Juglans regia L.). Acta Agr Univ. Pekinesis. 1986; 12(2):143-8.
Tulecke W, McGranahan G. Somatic embryogenesis and plant regeneration from cotyledons of walnut, Juglans regia L. Plant Sci. 1985;40(1):57–63.
Felaliev A. Morphogenesis Juglans regia in vitro. Ukr Bot Zh. 1990;47(3):85-7.
Boltivets VS, Piven NM. Induced embryoidogenesis in the culture tissue of Juglans regia L. Fiziologiia i biokhimiia kul't rasteniy. 1990; 22(4):409-13
Murashige T, Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol Plant. 1962;15(3):473–97.
Jay-allemand C, Cornu D, Macheix JJ, Chanteloup G. Caractérisation du rajeunissement du Noyer (Juglans sp.) par une étude spectrophotométrique globale du contenu polyphénolique. Ann For Sci. 1987;44(3):303–14.
Felaliev A. Physiological basis of micropropagation of walnut in vitro: Author. dis. ... Kand. biol. nauk. Dushanbe, 1990. 20 p.
