Structural and functional properties of hemoglobins in two fish species: Cyprinus carpio and Hypophthalmichtys molitrix
DOI:
https://doi.org/10.7124/bc.0002D3Abstract
Microheterogeneity of hemoglobins in two fish species: C. carpio and H. molitrix has been studied by isoelectrofocusing within the pH gradient of 6:8. Species differences of these hemoglobins are shown in isoelectric spectra and amino acid composition of electrophoretically homogeneous fractions. The affinity to oxygen of hemoglobins fractions has been determined.References
Dudarev VP. Role of hemoglobin in the mechanisms of adaptation to hypoxia and hyperoxia. Kiev: Naukova Dumka, 1979; 150 p.
Irzhak MI. hemoglobins and their properties. Moscow: Nauka, 1978; 235 p.
Starodub NF. Heterogeneous system of hemoglobin. Structural features, physico-chemical and functional properties of individual forms of hemoglobin and their role in the biochemical adaptation to the conditions of life. Usp Sovrem Biol. 1985; 99(3):385-400.
Drabkin DL. Spectrophotometric studies; the crystallographic and optical properties of the hemoglobin of man in comparison with those of other species. J Biol Chem. 1946;164(2):703-23.
Davis BJ. Disc electrophoresis. II. Method and application to human serum proteins. Ann N Y Acad Sci. 1964;121:404-27.
Azhitskiĭ GIu, Bagdasar'ian SN. Possibility of isolation of monomeric immunochemically pure serum albumin. Lab Delo. 1975;(12):712-4. PubMed
Troitskiĭ GV, Azhitskiĭ GIu, Malyi DD. Isoelectric focusing in a boroborate buffer-mannitol system (BBM). Vopr Med Khim. 1976;22(2):282-6.
Paskhina TS. Quantitative determination of amino acids on chromatograms with the aid of ninhydrin reaction. Biokhimiia. 1954;19(6):702-12.
Shorokhov IuA. Spectrophotometric method of determining oxyhemoglobin dissociation curves in a cuvette-desaturator. Fiziol Zh SSSR Im I M Sechenova. 1974;60(4):654-6.
Benesch RE, Benesch R, Yung S. Equations for the spectrophotometric analysis of hemoglobin mixtures. Anal Biochem. 1973;55(1):245-8.
Starodub NF, Nazarenko VI. Heterogeneous hemoglobin system. K.: Naukova Dumka, 1987; 199 p.
