Obtaining of the anti-sense cDNA construction of human low-density lipoprotein receptor
DOI:
https://doi.org/10.7124/bc.0002CCAbstract
The anti-sense cDNA construction of human low-density lipoprotein receptor (rLDL) was obtained. It includes the 5'-terminal fragment of cDNA rLDL and the murine metal-lothionein gene promoter. The construction may be used to obtain transgenic animals that are deficient of rLDL.References
Green PJ, Pines O, Inouye M. The role of antisense RNA in gene regulation. Annu Rev Biochem. 1986;55:569-97.
Brown MS, Goldstein JL. A receptor-mediated pathway for cholesterol homeostasis. Science. 1986;232(4746):34-47.
Palmiter RD, Norstedt G, Gelinas RE, Hammer RE, Brinster RL. Metallothionein-human GH fusion genes stimulate growth of mice. Science. 1983;222(4625):809-14.
Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
Calvin NM, Hanawalt PC. High-efficiency transformation of bacterial cells by electroporation. J Bacteriol. 1988;170(6):2796-801.
Hammer RE, Brinster RL, Rosenfeld MG, Evans RM, Mayo KE. Expression of human growth hormone-releasing factor in transgenic mice results in increased somatic growth. Nature. 1985 May 30-Jun 5;315(6018):413-6.
Dolzhanskaya NB, Mandelshtam MYu, Patkin EL, Kuznetsov AS, Vikhanskaya FL, Krutilina RI, Gaitskhoki VS. A model for genetic correction of family hypercholesterolemia. Biopolym Cell. 1990; 6(2):31-5.
