The influence of UV irradiation on the transfer of foreign genes into Ti plasmid of Agrobacterium tumefaciens via intermediate vectors
DOI:
https://doi.org/10.7124/bc.0002BFAbstract
The influence of UV irradiation of recipient A. tumefaciens cells on the frequency of the foreign gene transfer into Ti plasmid via intermediate vectors has been investigated. It has been established that the frequency of this process after transient irradiation of recipient cells is 10–100 times higher than the frequency observed without irradiation. This fact is suggested to be used as an effective method to increase the foreign gene transfer frequency into Ti plasmid if the intermediate vectors are applied for it.References
Leemans J, Shaw C, Deblaere R, De Greve H, Hernalsteens JP, Maes M, Van Montagu M, Schell J. Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells. J Mol Appl Genet. 1981;1(2):149-64.
Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature. 1983;303(5913):179–80.
Van Haute E, Joos H, Maes M, Warren G, Van Montagu M, Schell J. Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO J. 1983;2(3):411-7.
Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. New York: Cold Spring Harbor Lab, 1982; 545 p.
Zambryski P, Joos H, Genetello C, Leemans J, Montagu MV, Schell J. Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity. EMBO J. 1983;2(12):2143-50.
