Optimization of the β-lactamase TEM1 phage-dependent synthesis system in Escherichia coli cells
DOI:
https://doi.org/10.7124/bc.0002BEAbstract
The original system of the β-lactamase gene amplification has been worked out. Such a system can produce 6.0·105 units of β-lactamase activity per ml of cultural medium and has such an advantage as the accessible enzyme form.References
Hershfield V, Boyer HW, Yanofsky C, Lovett MA, Helinski DR. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974;71(9):3455-9.
Belder JB, Nguyen-Dist?che M, Houba-Herin N, Ghuysen JM, Maruyama IN, Hara H, Hirota Y, Inouye M. Overexpression, solubilization and refolding of a genetically engineered derivative of the penicillin-binding protein 3 of Escherichia coli K12. Mol Microbiol. 1988;2(4):519-25.
Georgiou G, Telford JN, Shuler ML, Wilson DB. Localization of inclusion bodies in Escherichia coli overproducing beta-lactamase or alkaline phosphatase. Appl Environ Microbiol. 1986;52(5):1157-61.
Moir A, Brammar WJ. The use of specialised transducing phages in the amplification of enzyme production. Mol Gen Genet. 1976;149(1):87-99.
Plasmids: A practical approach. Edited by K.G. Hardy. Oxford-Washington, DC: IRL Press, 1987; 180 pp.
Dagert M, Ehrlich SD. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979;6(1):23-8.
Sorochynskaja TV, Chernykh SI. Study of ?-lactamase tem1 synthesis in various escherichia coli strains infected by the phage ? bla with amber-mutations in different regulatory genes. Biopolym Cell. 1991; 7(1):101-7.
Chaikovskaia SM, Venkina TG. A modified iodometric method for the determination of penicillinase activity. Antibiotiki. 1962;7:453-6.
Gurevich AI, Babi? NI. The role of structural elements of ColE1-related plasmids in replication control. Bioorg Khim. 1987;13(2):213-7.
