Study of β-lactamase tem1 synthesis in various escherichia coli strains infected by the phage λ bla with amber-mutations in different regulatory genes
DOI:
https://doi.org/10.7124/bc.0002BAAbstract
The search of optimal conditions for phage-dependent supersynthesis of β-lactamase was undertaken. For this purpose the recombinant phage with alternatively orientated β-lactamase (bla) structure gene were obtained. It was stated, that phage λblaN–N-, λblaQ–R– derivates are obtained. The β-lactamase producing strain was selected. The maximal recovery of β-lactamase was obtained while Su0 E. coli W3101 strain was infected by λ-phage bla Q–R–.References
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