Acidic polypeptides as inhibitors of the repair of double-strand DNA breaks
DOI:
https://doi.org/10.7124/bc.0002A7Abstract
The ability of polypeptides consisting of aspartic and glutamic acids to inhibit the repair of double-strand DNA breaks induced in Y-irradiated Chinese hamster and HeLa cells was shown. A complete inhibiton of the double-strand DNA breaks repair was observed under the concentrations of 20 jimoles/1 (polyglutamic acid with molecular weight 2000–15000 daltons) and 100 umoles/1 (aspartylglutamic acid with moSccuar weight 1500-4500 daltons). Both polypeptides were low toxic at the given concentrations, their inhibitory effect being reversible.References
Cooper WJ, Cole A. Characterization studies of the rejoining of ?-particle and ?-ray induced double-strand acissions in mammalian DNA. Radiat Res. 1974; 59(1):262.
Bryant PE, Blöcher D. The effects of 9-beta-D-arabinofuranosyladenine on the repair of DNA strand breaks in X-irradiated Ehrlich ascites tumour cells. Int J Radiat Biol Relat Stud Phys Chem Med. 1982;42(4):385-94.
A. c. 1418339, C12 N 15. 00. Inhibitor of DNA double strand breaks repair. AI. Medvedev. Bull. Izobreteniy. 1988; (31): 121.
Medvedev AI, Mantsygin IuA, Revina GI, Veselova VA, Kublik LN. Effect of natural and synthetic acid polypeptides on normal and gamma-irradiated cells of the Chinese hamster. Radiobiologiia. 1978;18(6):821-8. Russian.
Medvedev AI, Revina GI, Kuzin AM. Strongly acid polypeptides as inhibitors of the repair of single-stranded DNA breaks induced by gamma irradiation. Dokl Akad Nauk SSSR. 1983;270(1):232-5.
Bradley MO, Kohn KW. X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution. Nucleic Acids Res. 1979;7(3):793-804.
Flerov GN, Barashenkov VS. Practical applications of heavy ion beams. Uspekhi fizicheskikh nauk. 1974; 114(2):351-73.
Adams RLP. Cell Culture for Biochemists. Elsevier/North-Holland Biomedical Press. 1980; 292 p.
