A computer-aided search for giant DNA-protein complexes
DOI:
https://doi.org/10.7124/bc.0002A0Abstract
Two simple programs to detect extensive spatial asymmetries of DNA were created. The programs were used to study the 2 u,m plasmid of Saccharomyces yeast. A nearly 600 bp. uninterrupted region of special spatial orientation of G-C base pairs was found covering the REP 3 locus. The anomaly was obvious when estimating the helix lead approx. 104.4 (but not at 1 ,10:0 or 10.6). It is supposed that the region corresponds to a giant non-nucleosomal DNA-protein complex supposedly of an «oozing» mechanism according to the classification by Ptashne (19*86). The complex is also indicated by some recent experimental results. The new programs can be used for searching giant DNA-protein complexes.References
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