Radiation-induced unscheduled synthesis of DNA and DNA polymerase activity at the nuclear matrix
DOI:
https://doi.org/10.7124/bc.00018CAbstract
At the nuclear matrix of UV- and Y-irradiated cells from Zajdela hepatoma the higher level of hydroxyurea-resistant DNA synthesis is observed in the initial (1.5-5 min) period of postradiation incubation in comparison with total nuclear DNA. But 1-β-D arabinofuranosylcytosine-resistant radiation-induced DNA synthesis is the same both in the nuclear matrix and in the intact nuclei of these cells. Both DNA polymerase α and DNA polymerase β are shown to participate in DNA synthesis at the nuclear matrix. DNA polymerase β is more strongly associated with the nuclear matrix of normal liver cells in comparison with nuclei of the regenerating liver. The poly-(ADP-ribose)synthetase activity is shown to be associated with the nuclear matrix. Under the activity inhibition the hydroxyurea-resistant DNA synthesis is, mainly, induced in the nuclear matrix.References
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