A method for selection of hybridomas, secreting monoclonal antibodies against tyrosyl-tRNA synthetase, based on monitoring of the enzymatic activity

Authors

  • T. A. Ribkinska Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • O. A. Vartanyan Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR Moscow, USSR Author
  • V. V. Filonenko Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • L. L. Sidorik Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • A. I. Kornelyuk Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • S. F. Beresten Engelhardt Institute of Molecular Biology, Academy of Sciences of the USSR Moscow, USSR Author

DOI:

https://doi.org/10.7124/bc.000285

Abstract

A new method for screening populations of hybrid cells secreting monoclonal antibodies (Mabs) against aminnacyl-tRNA synthetases (ARS) is developed as based on monitoring of the enzymatic activity of ARSes. The main advantage of this procedure stems from the fact that it permits using non-homogeneous ARS preparations both for immunization and for screening. While applying this procedure, hybridoma cell line T3 secreting Mabs against bovine tyrosyl-tRNA synthetase has been produced and characterized.

References

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Published

1990-07-20

Issue

Vol. 6 No. 4 (1990)

Section

Gene-Engineering Biotechnology