Isolation of seryl-tRNA synthetase from the animal liver by proximate method

Authors

  • O. I. Gudzera Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • L. L. Sidorik Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • I. V. Zolotukhina Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • M. A. Tukalo Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • G. Kh. Matsuka Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author

DOI:

https://doi.org/10.7124/bc.000264

Abstract

A proximate method to isolate seryl-tRNA synthetatse from the animal liver is described. It includes polyethylene glycole 6000 fractionation, chromatography on DEAE-Toyopearl 650 M, and chromatography on the FPLC system of Mono S and Mono Q sorbents. A yield of the purified enzyme was about 600 ng per 1 kg of liver. The enzyme is a structural dimer (oc2 type) with molecular weight of 120 kDa.

References

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Published

1990-03-20

Issue

Vol. 6 No. 2 (1990)

Section

Short Communications