Comparative studies in efficiency of expression of bacterial β-galactosidase in vivo by the immunofluorescence method
DOI:
https://doi.org/10.7124/bc.000249Abstract
The expression of some vector molecules encoding the synthesis of bacterial p-galactosidase in mouse liver hepatocytes 24 h after direct injection has been investigated. The advantages of immunofluorescence method with the microcytophotometry for rapid test of gene expression at the cell level are discussed.References
Coons AH, Leduc EH, Connolly JM. Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med. 1955;102(1):49-60.
An G, Hidaka K, Siminovitch L. Expression of bacterial beta-galactosidase in animal cells. Mol Cell Biol. 1982;2(12):1628-32.
O'Hare K, Benoist C, Breathnach R. Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase. Proc Natl Acad Sci U S A. 1981;78(3):1527-31.
Kostetsky IE, Shpilevaya SP, Likhacheva LI, Zharova LC, Irodov DM, Kirillova VS, Kordium VA. Expression of Escherichia coli?-Galactosidase in murine hepatocytes. Biopolym Cell. 1989; 5(2):51-8.
Rhodes JM, Blom J. Cytochemical localization of beta-galactosidase in resident and inflammatory peritoneal macrophages from C57BL mice. Histochemistry. 1986;86(2):159-64.
Zilber A. Immunological analysis. Moscow, Medicine, 1968; 185 p.
Ernst B, Tasman D. Immunology. Immunological Methods. Moscow, Mir, 1979; 373-413.
