Advantages of Z-gal during selection of recombinants on Lac phenotype

Authors

  • E. B. Paton Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author
  • A. G. Terentyev Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian SSR Kiev, USSR Author

DOI:

https://doi.org/10.7124/bc.0000DE

Abstract

Advantages of Z-gal use as a chromogenic substrate of (5-galactosidase, when selecting recombinants by Lac phenotype, are discussed.

References

Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103-19.

Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982;19(3):259-68.

Zinder ND, Boeke JD. The filamentous phage (Ff) as vectors for recombinant DNA--a review. Gene. 1982;19(1):1-10.

Dente L, Cesareni G, Cortese R. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 1983;11(6):1645-55.

Paton EB, Kroupskaya IV, ZhyvolouP AN. Unidirectional orientation of Escherichia coli rPlKAJL-rPoBC operon fragments on a multicopy PUC Plasmid. Biopolym Cell. 1989; 5(1):58-66.

Minton NP. Improved plasmid vectors for the isolation of translational lac gene fusions. Gene. 1984;31(1-3):269-73.

Paton EB, Kroupskaya IV, ZhyvolouP AN. Studies in the regulation of Escherichia coli rPlL gene exPression by the method of lacZ fusions in PNM481 plasmid. Biopolym Cell. 1989; 5(2):99-102.

Published

1989-07-20

Issue

Section

Short Communications