Ethidium bromide binding to polyriboguanylic acid
DOI:
https://doi.org/10.7124/bc.0000C6Abstract
The binding of ethidium bromide to polyriboguanylic acid has been investigated according to the character of changes in absorption spectra. The calculations have shown that the saturation stoichiometry at binding makes up one ethidium bromide molecule per 5 guanine bases with binding constant (5.2±0.8) -104 M-1 in the presence of 0.1 M NaCl and at room temperature.References
Cantor CR, Schimmel PR. Biophysical Chemistry. San Francisco, WH Freeman and Co., 1985; Vol. 3, 534 p.
Nelson JW, Tinoco I Jr. Intercalation of ethidium ion into DNA and RNA oligonucleotides. Biopolymers. 1984;23(2):213-33.
Aktipis S, Kindelis A. Optical properties of the deoxyribonucleic acid-ethidium bromide complex. Effect of salt. Biochemistry. 1973;12(6):1213-21.
Bresloff JL, Crothers DM. Equilibrium studies of ethidium--polynucleotide interactions. Biochemistry. 1981;20(12):3547-53.
Waring MJ. DNA modification and cancer. Annu Rev Biochem. 1981;50:159-92.
Babayan Y, Manzini G, Xodo LE, Quadrifoglio F. Base specificity in the interaction of ethidium with synthetic polyribonucleotides. Nucleic Acids Res. 1987;15(14):5803-12.
Saenger W. Principles of nucleic acid structure. New York: Springer, 1984; 556 p.
Zasedatelev AS, Gurskii GV, Vol'kenshtein MV. Theory of one-dimensional adsorption. I. Adsorption of small molecules on a homopolymer. Mol Biol. 1971;5(2):194-8.
McGhee JD, von Hippel PH. Theoretical aspects of DNA-protein interactions: co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous lattice. J Mol Biol. 1974;86(2):469-89.