Stability of secondary structure of the oligonucleotide substrates. The effect of Ecodam DNA-methylase
DOI:
https://doi.org/10.7124/bc.0000B6Abstract
Oligonucleotide complexes containing various defects in Ecodam methylase recognition site have been investigated for their stability. Partial duplex structure of the single-stranded 20-base long oligonucleotide containing a self-complementary hexanucleotide sequence is observed only below 5 °C. Other complexes are melting within the narrow temperature range of 22–31 °C in 30 mM of potassium-phosphate buffer, pH 7.8. Presence of Ecodam methylase results in an increase of complex melting point at least by 5 °C.References
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