Studies in possibility of expression of exogenic human insulin gene in cultured mammalian fibroblasts
DOI:
https://doi.org/10.7124/bc.0000A7Abstract
Expression of human insulin genomic gene is observed in human and murine fibroblasts. Protein-product (proinsulin or insulin) detected by ELISA is secreted by fibroblasts. The protein-product secreted level achieves its peak on 9th-10th days and may be expressed in 125-160 ng/ml of culture medium. The similar dependence of proinsulin / insulin secretion by human and murine fibroblasts is determined.References
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