Cardiospecific knockout of αE-catenin leads to violation of the neonatal cardiomyocytes maturation via β-catenin and Yap signaling
DOI:
https://doi.org/10.7124/bc.00096AKeywords:
heart maturation, αE-catenin, Wnt signaling, HIPPO signaling, cardiomyocyteAbstract
Aim. To study the αE-catenin gene function in neonatal cardiomyocytes proliferation and maturation. Methods. Conditional knockout approach, histological (H&E and acridine orange staining) and molecular genetics (qPCR) methods were used. Result. The hetero- and homozygous embryonic cardiospecific knockout of αE-catenin is associated with an increased level of neonatal cardiomyocytes proliferation and a decreased binucleated cells frequency. Knockout of αE-catenin leads to up-regulation of the β-catenin- and Yap-target genes expression (Axin2, c-Myc, Tcf-4, CyclinD1, Ctgf and Tnfrsf1b). Conclusion. αE-catenin is involved in the regulation of proliferation and maturation of neonatal cardiomyocytes via modulation of the activity of β-catenin- and Yap-dependent transcription.References
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