Spreading and proliferation of cultured rat bone marrow stromal cells on the surface of bioactive glass ceramics

Authors

  • V. V. Kiroshka Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author
  • O. V. Savvova National Technical University “Kharkiv Polytechnic Institute” 2, Kyrpychova str., Kharkiv, Ukraine, 61002 Author
  • Yu. O. Bozhkova Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author
  • I. V. Tamarina Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine 23, Pereyaslavskaya Str., Kharkiv, Ukraine, 61015 Author
  • A. I. Fesenko National Technical University “Kharkiv Polytechnic Institute” 2, Kyrpychova str., Kharkiv, Ukraine, 61002 Author

DOI:

https://doi.org/10.7124/bc.00093F

Keywords:

glass-crystalline materials, bone marrow stromal cells

Abstract

Aim. To study spreading profile, cytoskeleton organization and proliferation of bone marrow stromal cells upon cultivation on the glass-crystalline material (GCM) surfaces with different chemical composition and solubility. Methods. GCMs with different CaO: P2O5 ratios were used. Actin cytoskeleton in cells was visualized using fluorescent TRITC-conjugated phalloidin. Cell proliferation was studied using MTT test. Results. Cell cultivation on highly soluble B-series GCM (Ca/P=5) led to appearance of fibroblast-like cells; their actin cytoskeleton filaments were uniformly distributed within the cytoplasm. In this case, proliferation dynamics was similar to that under cultivation on plastic. Cultivation on A and C series of GCM with a reduced Ca/P (1.4–2.58) resulted in a decrease of cell spreading and their proliferation index (up to 2–3 times) relative to the control. Conclusions. The material solubility and Ca/P superficial ratio are the main factors, determining cell interaction with GCMs.

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Published

2017-02-28

Issue

Vol. 33 No. 1 (2017)

Section

Molecular and Cell Biotechnologies