Microcalorimetric studies in hydration of Poly(dA)-Poly(dT) and Poly(dG)-Poly(dC) polynucleotides

Authors

  • G. M. Mrevlishvili Department of Macromolecular Physics, State University of Tbilisi Tbilisi, USSR Author
  • V. M. Sokhadze Institute of Physics, Academy of Sciences of the Georgian SSR Tbilisi, USSR Author
  • G. Sh. Japaridze Institute of Physics, Academy of Sciences of the Georgian SSR Tbilisi, USSR Author
  • D. A. Tatishvili Institute of Physics, Academy of Sciences of the Georgian SSR Tbilisi, USSR Author
  • N. E. Yakobashvili Department of Macromolecular Physics, State University of Tbilisi Tbilisi, USSR; Institute of Physics, Academy of Sciences of the Georgian SSR Tbilisi, USSR Author

DOI:

https://doi.org/10.7124/bc.00003B

Abstract

The calorimetric data show: 1) poly (dA) •poly (dT) hydration is nat = 27.0 ± l M H2O/MBP at all values of relative humidity (RH); 2) poly (dG) • poly (dC) hydration at high values of RH is nGC = 16.0 ± 1 MH2O/MBP; 3) thermodynamic parameters of ice-water phasetransition in poly(dQ) poly (dC) solutions and gels are abruptly changed with a decrease of the total water content (in the RH range of 90–60 %; 4) poly(dG) • poly(dC) hyd-ration at low RH is /toe = 12.0 ± 1MH2O/MBP; 5) the observed hydration change of poly (dG) poly (dC) (by 4±1MH2O/MBP) points to the conformational transition of this polymer within the limits of double-stranded state during the dehydration.

References

Ivanov VI, Minchenkova LE, Minyat EE, Frank-Kamenetskii MD, Schyolkina AK. The B to A transition of DNA in solution. J Mol Biol. 1974;87(4):817-33.

Arnott S, Selsing E. Letter: The structure of polydeoxyguanylic acid with polydeoxycytidylic acid. J Mol Biol. 1974;88(2):551-2.

Leslie AG, Arnott S, Chandrasekaran R, Ratliff RL. Polymorphism of DNA double helices. J Mol Biol. 1980;143(1):49-72.

Dickerson RE, Drew HR, Conner BN, Wing RM, Fratini AV, Kopka ML. The anatomy of A-, B-, and Z-DNA. Science. 1982;216(4545):475-85.

McCall M, Brown T, Kennard O. The crystal structure of d(G-G-G-G-C-C-C-C). A model for poly(dG).poly(dC). J Mol Biol. 1985;183(3):385-96.

Rich A, Nordheim A, Wang AH. The chemistry and biology of left-handed Z-DNA. Annu Rev Biochem. 1984;53:791-846.

Dickerson RE, Drew HR. Structure of a B-DNA dodecamer. II. Influence of base sequence on helix structure. J Mol Biol. 1981;149(4):761-86.

Drew HR, Dickerson RE. Structure of a B-DNA dodecamer. III. Geometry of hydration. J Mol Biol. 1981;151(3):535-56.

Kopka ML, Fratini AV, Drew HR, Dickerson RE. Ordered water structure around a B-DNA dodecamer. A quantitative study. J Mol Biol. 1983;163(1):129-46.

Calladine CR, Drew HR. A base-centred explanation of the B-to-A transition in DNA. J Mol Biol. 1984;178(3):773-82.

Minchenkova LE, Schyolkina AK, Chernov BK, Ivanov VI. CC/GG contacts facilitate the B to A transition of DNA in solution. J Biomol Struct Dyn. 1986;4(3):463-76.

Kennard O, Cruse WB, Nachman J, Prange T, Shakked Z, Rabinovich D. Ordered water structure in an A-DNA octamer at 1.7 A resolution. J Biomol Struct Dyn. 1986;3(4):623-47.

Saenger W, Hunter WN, Kennard O. DNA conformation is determined by economics in the hydration of phosphate groups. Nature. 1986 Nov 27-Dec 3;324(6095):385-8.

Frank-Kamenetskii M. DNA structure: a simple solution to the stability of the double helix? Nature. 1986 Nov 27-Dec 3;324(6095):305.

Mrevlishvili GM. Low-temperature calorimetry of biological macromolecules. Usp fiz nauk. 1979; 128(2):274-312.

Andronikashvili EL, Mrevlishvili GM, Japaridze GS, Sokhadze VM, Tatishvili DA. Low-temperature calorimetry of biological macromolecule solutions. J polym sci. 2007;69(1):11–5.

Mrevlishvili GM. Ratio of natural DNA hydration to the GC content. Dokl Akad Nauk SSSR. 1981;260(3):761-4.

Mrevlishvili GM, Japaridze GS, Sokhadze VM, Chanchalashvili ZI, Tatishvili DA. Hydration of DNA and Possible Role of Water in the Transition between Right- and Left-Handed Double Helix. Water and Ions in Biological Systems. 1985;161–7.

Mrevlishvili GM. Heat Capacities of Biological Macromolecules. Thermodynamic Data for Biochemistry and Biotechnology. Springer Berlin Heidelberg; 1986;148–76.

Mrevlishvili G. M., Syrnikov Yu. P. Thermodynamic quantities of water-phase transition in biopolymer solutions of high concentration. . Stud. biophys. 1974. 43, N3:155-170.

Nishimura Y, Torigoe C, Tsuboi M. An A-form poly(dG).poly(dC) in H2O solution. Biopolymers. 1985;24(9):1841-4.

Sarma MH, Gupta G, Sarma RH. 500-MHz 1H NMR study of poly(dG).poly(dC) in solution using one-dimensional nuclear Overhauser effect. Biochemistry. 1986;25(12):3659-65.

Lesnik EA, Agranovich IM, Maslova RN, Varshavskiĭ IaM. Study of conformation characteristics of polydeoxyribonucleotides with non-random base sequence by slow 1H----3H exchange. Mol Biol (Mosk). 1987;21(2):422-7.

biopolym.cell-1989-5-1-cover.png

Downloads

Published

1989-01-20

Issue

Vol. 5 No. 1 (1989)

Section

Structure and Function of Biopolymers