Cloning and characterization of Dmλ5 clone containing type I insertions of Drosophila melanogaster ribosomal genes

Authors

  • R. P. Vashakidze Institute of Molecular Biology and Biological Physics, Academy of Sciences of the Georgian SSR Tbilisi, USSR Author
  • N. Z. Mzhavia Institute of Molecular Biology and Biological Physics, Academy of Sciences of the Georgian SSR Tbilisi, USSR Author

DOI:

https://doi.org/10.7124/bc.000242

Abstract

The 12.5 kb sequence of DNA containing distal fragment of type I ribosomal repeat insertion and repeating elements of A89 clone from D. melanogaster genomic library has been cloned. It has been shown that fragment of type I insertion in the cloned sequence may be flanked both by the unique nontranscribed sequences and a mobile genetic element.

References

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Dawid IB, Botchan P. Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer. Proc Natl Acad Sci U S A. 1977;74(10):4233-7.

Dawid IB, Long EO, DiNocera PP, Pardue ML. Ribosomal insertion-like elements in Drosophila melanogaster are interspersed with mobile sequences. Cell. 1981;25(2):399-408.

Vashakidze RP, Kolchinskiy AM, Tamarina NA et al. Study clone DmA89, representative of a new family of mobile dispersed genes Drosophila melanogaster. V All-Union symp. "Molecular mechanisms of genetic processes": Abstracts. of reports. Moscow, 1983; 13.

Kolchinskiy AM, Vashakidze RP, Ananiyev EV et al. Genes Drosophila melanogaster, encoding rich polyadenylated mRNA: cloning, localization and expression. Mol Biol (Mosk). 1985; 19(6):1569-77.

Kolchinsky AM, Vashakidze RP, Kupert EYu, Korochkin LI. Uneven distribution of cloned transcribed DNA sequences in polytene chromosomes of Drosophila melanogaster. Cell Differ. 1986;18(2):145-9.

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Published

1988-11-20

Issue

Section

Short Communications