Genetic transformation of potato (Solanum tuberosum L.) using a binary Agrobacterium tumefaciens vector with patatin promoter class I
DOI:
https://doi.org/10.7124/bc.000420Abstract
Kanamycin resistant plants of S. tuberosum L. (in vitro-grown) cv. Zarevo were obtained from tlie cocultivated microtubers with A. tumefaciens. A disarmed binary vector systems containing the neomycin phosphotransferase (NPTII) gene as selectable marker and chloramphenicol acetyltransferase (CAT), as a reporter gene, under control of new patatin promoter class I were utilized. In vitro grown minitubers discs were used as sources of explants to produce transgenic plants on selective medium containing 100 fig/1 kanamycin and CAT enzyme activities were detected.References
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