Protein-lipid and protein-protein interactions of Bcr PH domain
DOI:
https://doi.org/10.7124/bc.00077AKeywords:
Bcr-Abl, PH domain, mass spectrometryAbstract
Bcr is a partner of Abl in reciprocal translocation t(9;22) which leads to Philadelphia chromosome formation. In the present study we analyzed Bcr PH domain functions and determined lipid specificity of PH domain in vitro. The technical approach for determining PH domain protein-protein interactions using mass-spectrometry was developed. Interactions with SMC1, β-tubulin, zizimin1 and PLCε were confirmed in vitro and in vivo.References
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